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FAQs Frequently asked questions: 1. What regulations exist for animal research in New Zealand? New Zealand law mandates that researchers must apply to an Animal Ethics Committee (AEC) to gain approval for using animals in research, testing, and teaching. These AECs are also tasked with monitoring approved research activities. The composition of AECs includes a veterinarian, a scientist, a nomination of an approved organization (e.g., SPCA), and a layperson with no involvement in animal research. This diverse membership provides a broad perspective on animal welfare. The government, while not directly involved in AEC decision-making, regulates animal research by reviewing the codes of ethical conduct that AECs and researchers operate under, oversees these AECs and requires annual reporting from the organisations that have them. 2. Is cosmetic testing on animals allowed in New Zealand? Testing on animals for developing, making, or testing a cosmetic or an ingredient that is intended exclusively for use in a cosmetic is explicitly banned in New Zealand under the Animal Welfare Act 1999, 2015 amendment. 3. How are animals chosen for research? Animals are selected for research based on the specific needs of the study and the suitability of different species to provide relevant data. The selection process is governed by ethical considerations, aiming to use non-sentient, or non-living organisms where possible and to minimize the number of animals used. Researchers must demonstrate that no viable alternatives exist and that the potential benefits of the research justify the use of animals. 4. Are there alternatives to using animals in research? Yes, researchers actively seek alternatives to animal testing, such as cell-based models, computer modelling, and other technologies that can reduce or eliminate the need for animal use. This effort aligns with the Three Rs: Replacement of animals with non-animal methods, Reduction in the number of animals used, and Refinement of techniques to reduce impacts. 5. How can the public learn more about animal research? The public can learn more about animal research through various trusted sources, including animal welfare organizations, regulatory bodies, and research institutions. Many of these organisations support openness about use of animals in research and teaching as signatories to the Openness Agreement on Animal use in Research and Teaching in New Zealand. They provide educational resources online, offering insights into how animal research is conducted, regulated, and how it contributes to scientific and medical advancements. Engaging with these sources can provide a balanced view of the ethical considerations and the importance of animal research in certain contexts.

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Our People New Zealand Board Members: Emeritus Professor Pat Cragg (Chair), retired, formerly University of Otago (Joined 2020) Chair of the ANZCCART New Zealand Board since April 2020. Pat is a physiologist, and from undergraduate training, a zoologist; her research area covered cardiorespiratory function and control in health and disease; her breadth of teaching is typified as a co-editor of a long-standing physiology textbook. Prior to retirement in May 2018, she held appointments at the University of Otago, for instance as Head of Department of Physiology, Associate Dean Academic Health Sciences and Acting Dean of School of Biomedical Sciences. Pat was on the University's Animal Ethics Committee for 27 years and on the ANZCCART New Zealand Board for 18 years, with four years as Deputy Chair, as well as seven years as the NZ representative on the ANZCCART Australian Board. For many years Pat was Secretary of the Physiological Society of NZ and Chair of the Scientific Committee of the Otago Medical Research Foundation (OMRF). she now Chairs the OMRF Council. From mid-April 2019 to the end of January 2022, she returned from retirement to be the Acting Deputy Vice-Chancellor Academic at the University of Otago. Ian Saldanha (Deputy Chair), Biosecurity & Animal Welfare Advisor, Cawthron Institute (Joined 2021) I have been involved in the lab animal industry for over 10 years. Before taking up a position at the Cawthron Institute in Nelson, I was the Head of the Animal facility at the Malaghan Institute in Wellington. This is where my passion for working with animals grew. During this time, I served on the executive committee for Australia New Zealand Laboratory Animal Association (ANZLAA) for a few years where I used this opportunity to support those that work in the animal science field and meet others from the industry. One of the highlights in my career was getting the opportunity to travel the world, visit other animal facilities, and gain an understanding about how they operate. Outside my profession I have a love for the outdoors in particular running. I enjoy travelling, meeting new people, and of course spending time with my family. Dr Arnja Dale, Chief Scientific Officer, Royal New Zealand SPCA (Joined 2016) Arnja has over 20 years working in the field of animal welfare science in New Zealand and overseas. Arnja is the Chief Scientific Officer at SPCA New Zealand. Prior to joining SPCA, Arnja was a Senior Lecturer in Animal Welfare Science, Ethics and Law, leading numerous research projects, and also led the animal welfare investigations training programme at Unitec. She is a current member of the ANZCCART NZ, the National Animal Welfare Advisory Committee (NAWAC) and the National Animal Ethics Advisory Committee (NAEAC). Arnja is passionate about animal welfare science and changing the hearts and minds of the next generation through evidence-based education initiatives. Arnja lives in Auckland with her husband, 3 children, and her dog and cat.. Susan Doohan, Animal Welfare Officer, AgResearch (Joined 2024) Susan Doohan is a Massey trained veterinarian working as an Animal Welfare Officer based at AgResearch Grasslands Campus for the last 7 years. From an initial background in mixed practice, with a later focus on dairy cattle production, she enjoys getting to interact with inspiring research on a daily basis. Further education has included Massey postgraduate courses, and gaining Australian and New Zealand College of Veterinary Scientists membership in Animal Welfare in 2021. Working with technicians, farm staff, and scientists from varied backgrounds and skill levels, to train them to perform research and husbandry manipulations on production species, is a strong area of interest, alongside implementation of the 3R’s. Dr Nadia Mitchell, Senior Lecturer, Department of Agricultural Sciences, Lincoln University (Joined 2025) I am a neuroscientist at Lincoln University, where I lead the ovine Batten disease research programme. My research explores translational brain- and eye-directed gene therapies in sheep, and preclinical data from my laboratory has led to the FDA clearance of two gene therapy products and supported human clinical trials for CLN5 Batten disease and Frontotemporal Dementia in collaboration with industry partners. I am a passionate advocate for sustainable and ethical animal research and as an educator, my role is to instil a deep understanding of, and commitment to, animal welfare practices in research and applied farming settings. Outside of work, I love to run, walk and explore Aotearoa New Zealand with my friends and family. Dr Mike Pankhurst, Senior Lecturer, Department of Anatomy, University of Otago (Joined 2024) Mike is reproductive biologist who specialises in the biology of the ovary and female infertility. Mike began research with animals nearly 20 years ago at the University of Tasmania during his PhD studies examining tissue repair following brain injury. Since 2011, Dr Pankhurst has been investigating female reproduction and fertility at the University of Otago, initially as a postdoctoral fellow and now as Senior Lecturer in the Department of Anatomy. Mike’s research team conducts experiments with mouse models but also makes use of alternative techniques such as human (blood) samples, tissue discarded from abattoirs, mathematical models, cell culture, protein-based research and re-analysis of historic archived tissues (microscope slides) using new technologies. Mike is passionate about using a broad range of techniques because this helps to reduce the number of experiments involving animals but also increases the breadth of knowledge gained by research. Mike is the former chair of the Animal Users Group, and a current member of the Animal Practices and Compliances Steering Group, at the University of Otag o. Stacey Parbhu, Animal Welfare Science Manager, Ministry for Primary Industries (Joined 2023) Stacey is currently leading the Animal Welfare systems team at MPI. As part of this role, she works alongside the National Animal Ethics Advisory Committee (NAEAC) and a team of technical and science advisors to ensure good governance to support RTT activities across New Zealand. She has been active in the care of companion animals, research animal husbandry, animal project management and animal facility management in a variety of positions since 2007, including AgResearch and Massey University. In 2017, Stacey moved to Te Herenga Waka | Victoria University in Wellington, to operate their brand-new small animal facility, eventually becoming the facility manager. In 2024 Stacey finalised her master’s thesis which utilised a mouse IVF model, providing her a greater understanding of the challenges and complexities many organisations will encounter with animal replacement techniques ANZCCART Fellow (observer) - vacant The ANZCCART Fellow is an ANZCCART board observer position for early stage researchers, to both give early stage researchers an opportunity for ANZCCART board experience, but also to inform the Board with their perspectives. Previous ANZCCART Fellows were: 2023-2025: Morgan Heslop, doctoral student, Massey University 2021-2023: Essie Van Zuylen, Postdoctoral Researcher, University of Canterbury ANZCCART NZ EXECUTIVE OFFICER Dr Marc Rands (Joined 2014) I was born in Lower Hutt and my father was a pākehā and my mother Canadian. My family moved to the UK when I was eight, and I was schooled in London, before completing a degree in Marine and Environmental Biology at the University of St. Andrews in Scotland, and a doctorate from the University of Oxford (Linacre College) studying sea anemones with symbiotic algae. I met my wife at university in Scotland, and we lived in Perth (Scotland) with our two sons while undertaking work at the Institute of Aquaculture at the University of Stirling, then working at the Scottish Higher Education Funding Council in Edinburgh, before moving to the Royal Society of Edinburgh in 1998. In 2010, I took up a position at the Royal Society of New Zealand, where I worked on supporting the Society’s expert advice, and then supporting the Society's Academy. As the ANZCCART NZ Executive Officer, I work with the Board supporting the responsible use of animals in research and teaching.

Animal welfare What is animal welfare and whose welfare is considered? Animals are recognized as sentient beings in New Zealand law. This means they have sufficiently complex nervous systems to support flexible and adaptive behaviour and, importantly, a range of different experiences and feelings that they can interpret as good/positive or bad/negative. In New Zealand, animals considered to be sentient are mammals, birds, reptiles, amphibians, fish, octopus, squid, crabs, lobsters and crayfish (see Information about animal research in NZ for more information). The welfare of an animal depends on its mental state – the negative or positive experiences it is having at a particular point in time. Negative experiences might include pain, discomfort, breathlessness, nausea, fear, anxiety, hunger, thirst and even loneliness, frustration or boredom, depending on the kind of animal. Good experiences might include feelings of pleasure, comfort, safety and companionship, again depending on the kind of animal. The animal’s mental state is influenced by its perception of both its physical health and its environment. How can we understand an animal’s welfare state to make improvements? Often in research, it is necessary to perform procedures that may cause animals to experience negative states like fear, pain, nausea, or breathlessness. In addition to compromise during procedures, animals may also experience compromise in their home environment, during day-to-day husbandry and handling, preparation for, as well as recovery from, the procedure. An animal may be more likely to experience welfare compromise because of its age, physical state, particular type or breeding. When using animals in research, testing, and teaching, we have a responsibility to promote good welfare, meaning minimizing welfare compromise due to negative experiences and enhancing welfare by providing opportunities for positive experiences. The people responsible must consider the ways that their use of, or interaction with, the animals may impact their welfare. Another way to think about this is to ask “What mental states will the animal experience in this situation, in this physical condition or due to this procedure?”. We can understand the degree to which welfare is compromised by evaluating the quality, likelihood, severity/intensity and duration of any and all negative experiences; we do this using observable or measurable indicators of mental states, including behavioural, physiological and neurophysiological measurements. Likewise, we can understand the degree of welfare enhancement, primarily by evaluating animal’s behavioural responses to opportunities that arise or we provide. One model we can use to organize investigations of welfare and the evidence for an animal’s mental experiences and its overall welfare state is the Five Domains Model. This is an internationally recognised welfare assessment framework developed in New Zealand. It reflects modern understanding of animal welfare by breaking down the factors that influence welfare into four categories (Domain 1 Food and water; Domain 2 Physical environment; Domain 3 Health and physical status; Domain 4 Behavioural interactions) and includes the associated mental experiences in a fifth category (Domain 5 Mental states). With care, the Model can be used for any species in any context. It allows the user to apply their own knowledge of the animal species to identify potential sources of welfare compromise and opportunities for welfare enhancement. How can we improve the welfare of animals used in RTT? The Three Rs The Three Rs provide guidance on ways to minimize welfare compromise for animals used in RTT by avoiding animal use altogether (Replacement), reducing the number of animals that might experience welfare compromise whilst still achieving the purpose of having used them (Reduction) or reducing the likelihood, severity and/or duration of negative mental experiences arising due to the ways animals are used and managed (Refinement). Providing opportunities for positive experiences, while continuing to minimise negatives, is another way to refine animal use. For more information about the Three Rs see NC3Rs . Minimizing welfare compromise (Refinement) depends on what the problem is for the animal. Importantly, animals must be conscious to be having any mental experiences, including pain, so using general anaesthesia to make an animal unconscious is one way to reduce welfare compromise during a procedure. Pain can also be alleviated or reduced by using drugs that block or reduce pain signals (local anaesthetics or analgesics). However, pain-relieving drugs won’t reduce hunger, thirst, breathlessness, nausea, fear or loneliness. So, the remedy must be appropriate for the problem, and the effectiveness of the intervention should be evaluated using methods like those described above. For example, fear due to human-animal interactions can be reduced by avoiding or refining handling procedures, while anxiety might be relieved by manipulating animal housing or social groupings. Likewise, such changes in animal housing and management can provide animals opportunities for positive experiences. Promoting these feelings wherever possible can enhance the welfare of animals, as well as making them easier to handle and work with. Resources for minimizing welfare compromise: Beausoleil, N., & Mellor, D. (2015). Introducing breathlessness as a significant animal welfare issue. New Zealand Veterinary Journal , 63 (1), 44–51. https://doi.org/10.1080/00480169.2014.940410 Resources for enhancing welfare: ·Baumans, V. (2005). Environmental Enrichment for Laboratory Rodents and Rabbits: Requirements of Rodents, Rabbits, and Research. ILAR Journal , 46 (2), 162–170. https://doi.org/10.1093/ilar.46.2.162 Rault, J.-L., Waiblinger, S., Boivin, X., & Hemsworth, P. (2020). The Power of a Positive Human–Animal Relationship for Animal Welfare. Frontiers in Veterinary Science , 7 . https://www.frontiersin.org/articles/10.3389/fvets.2020.590867 Resources for understanding animal welfare and its assessment: Mellor, D. J. (2017). Operational Details of the Five Domains Model and Its Key Applications to the Assessment and Management of Animal Welfare. Animals, 7(8), Article 8. https://doi.org/10.3390/ani7080060 Mellor, D. J., Beausoleil, N. J., Littlewood, K. E., McLean, A. N., McGreevy, P. D., Jones, B., & Wilkins, C. (2020). The 2020 Five Domains Model: Including Human–Animal Interactions in Assessments of Animal Welfare. Animals, 10(10), 1870. https://doi.org/10.3390/ani10101870

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